chanelink logo
CHANELINK
Deutsch

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

122
2024-01-23 14:07:18
Übersetzung anzeigen

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

Ähnliche Empfehlungen

  • Innovating Photonics: Lithium Tantalate Provides Power for the Next Generation of Optoelectronic Circuits

    The new photonic integrated circuit technology based on lithium tantalate has improved cost efficiency and scalability, making significant progress in the fields of optical communication and computing.The rapid development of photonic integrated circuits (PICs) has revolutionized optical communication and computing systems, combining multiple optical devices and functions on a single chip.For deca...

    2024-05-14
    Übersetzung anzeigen

  • Lumibird, a well-known French optoelectronics company, increased its lidar production capacity by 16% year-on-year and was boosted by strong market demand

    On July 24, Lumibird, a well-known French optoelectronics company, released its latest semi-annual report. In the first half of the year, Lumibird's revenues were 97.2 million euros, up 16 percent from the same period last year. Of this, the Optoelectronics division contributed 45.9 million euros and the remaining 51.3 million euros came from its medical division. In the second quarter (Q2) ended ...

    2023-08-04
    Übersetzung anzeigen

  • TRUMPF machine cooler saves 50 percent energy

    Ditzingen, 05. March 2025 – At its in-house exhibition INTECH, high-tech company TRUMPF is showcasing a new cooler for its laser cutting machines. The new unit is capable of reducing energy consumed during the cooling process and uses fifty percent less energy than conventional solutions. Unlike conventional coolers, the main components of this new solution— such as pumps, fans and compressors— ar...

    03-14
    Übersetzung anzeigen

  • Intel: Has acquired most of ASML's NA extreme ultraviolet lithography equipment in the first half of next year

    According to Korean media reports, Intel has acquired most of the high numerical aperture (NA) extreme ultraviolet (EUV) lithography equipment manufactured by ASML in the first half of next year.ASML plans to produce 5 high NA EUV lithography equipment this year, all of which will be supplied to Intel.They stated that ASML has an annual production capacity of approximately 5-6 High Numerical Apert...

    2024-05-21
    Übersetzung anzeigen

  • Set a new world record! Optical crystals as thin as cicada wings increase energy efficiency by over a hundred times

    On quartz sheets, the angular rhombic boron nitride crystals with a thickness of only 1 to 3 microns are as thin as cicada wings, but their energy efficiency is 100 to 10000 times higher than traditional optical crystals. At the opening ceremony of the 2024 Zhongguancun Forum Annual Conference held on April 25th, the world's thinnest known optical crystal was listed as one of the top ten technolog...

    2024-04-26
    Übersetzung anzeigen