chanelink logo
CHANELINK
Français

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

145
2024-03-29 15:03:26
Voir la traduction

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Recommandations associées

  • The research results on the implementation of micro active vortex laser using laser nanoprinting technology are published in Nano Letters

    IntroductionVortex beams carrying orbital angular momentum (OAM) are widely used for high-throughput optical information multiplexing, and achieving on chip, small-scale vortex lasers is crucial for promoting the industrial implementation of vortex light reuse technology. Recently, Gu Min, an academician of Shanghai University of Technology, and Fang Xinyuan, an associate professor of Shanghai Uni...

    2023-10-16
    Voir la traduction

  • BLM Group launches a new LT12 laser tube cutting system

    Recently, BLM Group in the United States has launched a new LT12 laser tube system, which performs well in cutting light and heavy pipes and profiles, and can handle materials with a diameter of up to 305 millimeters.According to the company, compared to other similar machines, the LT12 laser tube system reduces cutting time by up to 55% when cutting materials with the same maximum diameter, signi...

    2024-04-18
    Voir la traduction

  • The construction of Hefei Advanced Light Source Project held a launch ceremony, expected to be completed and released in 5 years

    Recently, in the Future Science City of Hefei City, Anhui Province, the National Major Science and Technology Infrastructure Project and Supporting Projects of Hefei Advanced Light Source announced the start of construction, with a planned land area of approximately 656 acres. The first phase of the project is expected to be completed by September 2028.After completion, it will become an internati...

    2023-09-23
    Voir la traduction

  • The application of lasers in material processing has driven industrial progress in Santa Catalina state

    Laser material processing has been widely used in advanced industries, ranging from designing and producing lightweight, ultra wear-resistant parts and equipment with complex geometric shapes to repairing damaged or worn components through technologies such as 3D printing of deposited metal powders or deposits.Use laser pulses for surface treatment to prevent fatigue. But the impact of such techno...

    2023-09-26
    Voir la traduction

  • EO Technologies from South Korea enters the glass substrate processing market

    Recently, EO Technologies, a well-known semiconductor laser processing equipment manufacturer in South Korea, is emerging in the glass substrate processing market.It is understood that EO Technologies is entering the glass substrate TGV market based on its UV laser drilling equipment originally used in PCB substrate technology. TGV technology is the core process for drilling holes inside glass sub...

    2024-06-18
    Voir la traduction