English

New super-resolution microscopy imaging technology: rapid imaging of neurons

197
2024-12-04 14:16:07
See translation

The research group led by Wang Kai from the Center for Excellence in Brain Science and Intelligent Technology of the Chinese Academy of Sciences has published a research paper titled "Super solution imaging of fast morphological dynamics of neurons in eating animals" online in Nature Methods. The team has developed a new type of super-resolution microscopy imaging technology, which solves the two major technical problems of background noise interference and motion artifacts. It can perform super-resolution optical imaging and analysis of the rapid dynamics of neurons in awake animal brains, providing a new tool for exploring the basis of neuronal burst plasticity in animal learning processes.

In recent years, the newly developed super-resolution optical microscopy imaging technology can break through the optical diffraction limit. At present, due to multiple technical difficulties, super-resolution fluorescence microscopy is mostly used for ex vivo cell and brain slice research, and there is no technology that can super-resolution analyze the structure and function of synapses in normal physiological and behavioral states in awake animals. Therefore, the development of new super-resolution optical imaging technologies applicable to conscious animals is at the forefront of neuroscience and optical imaging technology.

The team proposed the Multi Mode Reuse Structured Light Illumination Super Resolution Microscopy Imaging Technology (MLS-SIM). The key innovation of MLS-SIM is the proposal to quickly switch between different line illumination modes in a single line scan imaging process to obtain super-resolution information in three directions, and to propose a new theoretical framework for super-resolution reconstruction to achieve accurate and efficient super-resolution image reconstruction. Under linear fluorescence excitation mode, MLS-SIM can perform continuous imaging of the micro dynamics of dendritic spines and axonal terminals in the cortex of conscious mice with a lateral resolution of 150 nanometers for thousands of frames, and the speed can reach several frames per second; Can tolerate sample motion of 50 microns per second without affecting its super-resolution imaging performance. Furthermore, nonlinear fluorescence excitation can be achieved through picosecond pulse laser, and nonlinear MLS-SIM can increase the lateral resolution to about 100 nanometers while maintaining the same tolerance for sample motion.

MLS-SIM fills the gap in imaging of awake animals using super-resolution microscopy and addresses the shortcomings of previous techniques in imaging time and photobleaching characteristics of live animals, opening up prospects for in vivo microscopic research. Using this technique, the study validated the rapidly changing spike dynamics on neuronal dendritic spines and axon terminals in the awake mouse brain, and quantified the microscopic rapid dynamic changes in neurons during the awake sleep cycle. At the same time, this technology achieved dual color super-resolution simultaneous imaging and analyzed the relationship between the microstructure of PSD-95 protein aggregates and dendritic spine formation. In the dual color imaging experiment, it was found that there are many dynamic small protrusions in the dendritic backbone. The size of this protruding structure is often smaller than the analytical capability of traditional two-photon imaging technology, and can only be dynamically analyzed through super-resolution imaging. Dynamic observation shows frequent small protrusion formation near PSD-95 clusters on dendritic backbones. Furthermore, through statistical analysis of the dynamics over a period of time, it was found that there is a significant co localization phenomenon between PSD-95 clusters on dendritic backbones and small protrusions on the backbones. This newly discovered small protrusion structure and its co localization with PSD-95 may imply a cellular mechanism for dendritic spine formation, providing new evidence for future synaptic plasticity research.


Application of MLS-SIM in cortical super-resolution imaging of conscious mice


This technology enables long-term and large-scale imaging and analysis of subcellular micro dynamics of neurons and other cells in the physiological state of conscious animals, laying the foundation for the application of super-resolution imaging in the field of neuroscience and providing new tools for neuroscience research.

Source: Opticsky

Related Recommendations
  • The physicist who built the ultrafast "attosecond" laser won the Nobel Prize

    Pierre Agostini, Ferenc Krausz, and Anne L'Huillier won the award for their ultra short optical pulses, which made close research on electrons possible.Ferenc Klaus, Anne Lullier, and Pierre Agostini (from left to right)Image sources: BBVA Foundation, Kenneth Ruona/Lund University, Ohio State UniversityThis year's Nobel Prize in Physics was awarded to three physicists - Pierre Agostini of Ohio St...

    2023-10-09
    See translation
  • The Asia Photonics Expo will be held in Singapore from February 26th to 28th, 2025

    The Asia Photonics Expo (APE), as an internationally leading comprehensive brand promotion and business negotiation platform for optoelectronics, will be grandly held from February 26 to 28, 2025 at the L1 exhibition hall of the Sands Expo&Convention Centre in Singapore. As the top event in the field of optoelectronics, APE Asia Optoelectronics Expo will focus on cutting-edge innovative techno...

    01-03
    See translation
  • NUBURU Announces Second Next Generation Blue Laser Space Technology Contract with NASA

    NUBURU, the leading innovator of high-power and high brightness industrial blue laser technology, announced today that it has been awarded a second phase contract worth $850000 by the National Aeronautics and Space Administration (NASA) to advance blue laser power transmission technology as a unique solution that significantly reduces the size and weight of equipment required for lunar and Martian...

    2024-05-13
    See translation
  • IPG Photonics has unveiled a new dual-beam laser with single-mode core power at the Novi Battery Show in Michigan

    IPG Photonics Corporation, a global leader in fiber laser technology, will highlight new and innovative laser solutions at the Battery Show from September 12 to 14, 2023 in Novi, Michigan, USA.The IPG booth will include industry-leading fiber laser sources and automated laser systems for electric vehicle battery welding applications.New laser technology pushes the limits of battery welding speedTo...

    2023-09-12
    See translation
  • POSTECH launches a new type of fiber optic fusion splicer FS-23 series

    FOSTEC announced on the 8th that a new type of fiber fusion splicer has been launched. The newly launched FS-23 series is a fusion splicer that can be used for fiber optic operations in long-distance optical networks and CCTV optical networks. It not only has a small size and light weight, but also has a sturdy design and a long-lasting battery, which can provide high-precision performance.A perso...

    2024-01-08
    See translation