chanelink logo
CHANELINK
Nederlands

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

118
2024-01-23 14:07:18
Bekijk vertaling

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

Gerelateerde aanbevelingen

  • Based on Transform Optics: Realizing an Ideal Omnidirectional Invisible Cloak in Free Space

    A team led by Professor Ye Dexin and Professor Chen Hongsheng from Zhejiang University, as well as Professor Yu Luo from Nanyang University of Technology, conducted practical research on full parameter transformation optical devices. The research team has designed and implemented an all parameter omnidirectional invisibility cloak based on the theory of linear transformation optics and omnidirecti...

    2024-04-29
    Bekijk vertaling

  • Dazu Photonics launched the third generation of high power fiber laser successfully increased the product power to 50kW

    In recent years, with the vigorous development of new energy and other industries, the improvement of environmental awareness and the increasing demand for new applications, the demand for fiber lasers in intelligent manufacturing is increasing, and the demand for power is also increasing, and high-power fiber lasers can significantly improve production efficiency and are widely sought after by th...

    2023-09-02
    Bekijk vertaling

  • University of Science and Technology of China Reveals High Precision Planarity Measurement of Cryogenic Arrays

    Professor Wang Jian, Deputy Chief Designer of the Low Temperature Array High Precision Planeness Survey Wide Area Sky Survey Telescope (WFST) announced by the University of Science and Technology of China, and teacher of the State Key Laboratory of Nuclear Detection and Nuclear Electronics, School of Physics, University of Science and Technology of China, is a research team of the Chinese Academy ...

    2023-08-14
    Bekijk vertaling

  • Scientists achieve extremely short laser pulses with a peak power of 6 terawatts

    RIKEN's two physicists have achieved extremely short laser pulses with a peak power of 6 terawatts (6 trillion watts) - roughly equivalent to the power generated by 6000 nuclear power plants. This achievement will contribute to the further development of attosecond lasers, for which three researchers were awarded the Nobel Prize in Physics in 2023. This study was published in the journal Nature Ph...

    2024-04-22
    Bekijk vertaling

  • Tsinghua University makes progress in the field of pre sensing optical computing

    In the era of the Internet of Things, visual image sensors, as key devices in the intelligent society, are embedded in various devices such as mobile communication terminals, smart wearable devices, automobiles, and industrial machines. With the continuous expansion of applications, higher requirements have been put forward for the system power consumption, response speed, safety performance, and ...

    2024-08-05
    Bekijk vertaling