chanelink logo
CHANELINK
Tiếng Việt

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

145
2024-03-29 15:03:26
Xem bản dịch

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Đề xuất liên quan

  • Sivers Photonics has received a $1 million order for advanced optical sensing products in fields such as LiDAR and industrial applications

    Sivers Semiconductors AB announced that its subsidiary Sivers Photonics has received a new order worth $1 million for advanced optical sensing products from three customers in the fields of LiDAR, Medical, and Industrial.In the first half of the fourth quarter of 2023, new orders were received from several US clients, which will lead to the manufacturing of advanced lasers and optical amplifiers f...

    2023-11-30
    Xem bản dịch

  • NASA's laser reflector instrument helps to accurately locate Earth measurements

    The most famous use of GPS satellites is to help people understand their location, whether it is driving cars, ships or planes, or hiking in remote areas. Another important but little-known use is to distribute information to other Earth observation satellites to help them accurately locate measurements of our planet.NASA and several other federal agencies, including the US Space Force, the US Spa...

    2023-12-12
    Xem bản dịch

  • Processing application of ultrafast laser on bulk metallic glass

    Recently, an international research team led by Professor Zhang Peilei from the School of Materials Science and Engineering at Shanghai University of Engineering and Technology published a review paper titled "Research status of femtosecond lasers and nanosecond lasers processing on bulk metallic glasses (BMGs)" in the renowned journal Optics&Laser Technology in the field of optics and lasers....

    2023-09-18
    Xem bản dịch

  • Korean laser company AP Systems establishes new AVP equipment division

    Recently, AP Systems, a well-known laser manufacturer in South Korea, established a new AVP equipment division for the advanced packaging field. This business unit will focus on laser equipment required for advanced packaging processes of high bandwidth memory (HBM).AP Systems is a subsidiary of APS Group, mainly focused on the fields of display and semiconductor laser processing equipment. It foc...

    01-15
    Xem bản dịch

  • Investing nearly £ 520 million, this synchrotron light source in the UK will be upgraded

    Recently, the UK's national synchrotron "Diamond Light Source" announced an investment of nearly £ 520 million ($648.3 million) to implement three new flagship beam lines and upgrade existing beam lines. This comprehensive upgrade will be delivered by 2030.The Department for Science, Innovation, and Technology and the biomedical charity Wellcome jointly approved the facility upgrade project,...

    2023-09-27
    Xem bản dịch