chanelink logo
CHANELINK
简体中文

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

147
2024-03-29 15:03:26
查看翻译

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

相关推荐

  • Laser based deformation may lead to self optimized aircraft wings

    Due to advances in materials science by Stockholm researchers, changing the shape during flight to better handle airflow passing through its aircraft wings may be imminent. The trick involves the melting and drilling capabilities of lasers.Researchers from KTH Royal Institute of Technology in Stockholm, Sweden conducted experiments on paraffin. Using the 2D version of the material, they were able ...

    2024-01-18
    查看翻译

  • The Key Role of Laser Pointing Stability in the Application of Lithography Systems

    Lithography is one of the core processes in semiconductor manufacturing, and extreme ultraviolet lithography technology, as a new generation lithography technology, is also in a rapid development stage. The basic principle is to use photoresist (also known as photoresist) to form corrosion resistance due to photochemical reactions after being photosensitive, and to engrave the patterns on the mask...

    2024-07-02
    查看翻译

  • Shanghai Optical Machinery Institute has made progress in high-efficiency optical parametric amplification technology

    Recently, a joint research team composed of Sun Meizhi, associate researcher of the High Power Laser Physics Joint Laboratory of the Chinese Academy of Sciences Shanghai Institute of Optics and Precision Mechanics, and Tu Xiaoniu, associate researcher of the Chinese Academy of Sciences Shanghai Institute of Silicate, proposed a new configuration of cross Fabry Perot intracavity optical parametric ...

    2024-07-11
    查看翻译

  • Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

    Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause ...

    2024-01-23
    查看翻译

  • Making Infrared Light Visible: New Equipment Utilizes 2D Materials to Convert Infrared Light

    Infrared imaging and sensing technology can be used in various fields, from astronomy to chemistry. For example, when infrared light passes through a gas, sensing changes in light can help scientists identify specific properties of the gas. The use of visible light may not always achieve this sensing.However, existing infrared sensors are bulky and inefficient. In addition, due to the use of infra...

    2024-06-24
    查看翻译