chanelink logo
CHANELINK
English

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

1058
2024-01-23 14:07:18
See translation

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

Related Recommendations

  • Swiitol Launches E24 Pro: A Breakthrough in Laser Engraving Technology

    In order to completely change the world of laser engraving, Swiitol has launched the E24 Pro, a 24W integrated laser engraving machine with cutting-edge features and functions. The Swiitol E24 Pro showcases an innovative integrated structure laser engraving machine made of durable aluminum alloy. It is worth noting that the device can be used out of the box without installation, providing users wi...

    2023-11-23
    See translation

  • Laser direct writing technology for preparing micrometer scale heatable graphene de icing and anti icing surfaces broadens the preparation method of new de icing and anti icing devices

    Research backgroundIn transportation, industrial production, and practical life, icing often brings great troubles, and the most serious is that during the flight of an aircraft, key components once frozen will seriously affect navigation safety.The traditional passive deicing and anti icing strategies for aircraft, such as mechanical vibration and anti freezing liquids, have problems such as inco...

    2023-10-16
    See translation

  • Scientists achieve extremely short laser pulses with a peak power of 6 terawatts

    RIKEN's two physicists have achieved extremely short laser pulses with a peak power of 6 terawatts (6 trillion watts) - roughly equivalent to the power generated by 6000 nuclear power plants. This achievement will contribute to the further development of attosecond lasers, for which three researchers were awarded the Nobel Prize in Physics in 2023. This study was published in the journal Nature Ph...

    2024-04-22
    See translation

  • Luxiner launches LXR ultra short pulse laser platform

    Luxiner, the global leader in laser technology, has launched LXR ® The ultra short pulse (USP) laser platform is a revolutionary leap in industrial laser processing. The LXR platform provides unparalleled performance, versatility, and reliability, making significant progress in burst mode processing. Micro Miracle MasterThe world of miniaturization is flourishing due to the continuous improvemen...

    2024-06-11
    See translation

  • The Institute of Physics, Chinese Academy of Sciences has made significant progress in the research of lithium niobate nanooptics

    In recent years, breakthroughs in the preparation technology of lithium niobate single crystal thin films have greatly promoted the important application of lithium niobate crystals in micro nano optical devices such as optical metasurfaces. However, the high hardness and inactive chemical properties of lithium niobate crystals pose significant challenges to micro nano processing; In addition, con...

    04-15
    See translation