chanelink logo
CHANELINK
English

Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

885
2024-01-23 14:07:18
See translation

Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause noise and distortion in the original image, which may affect the quality of the final SR image.

Therefore, for SR microscope developers and users, having a reliable method to quantify reconstruction quality is crucial. Due to the improved distinguishability of SR imaging, it is necessary to conduct a thorough evaluation, but existing tools are often insufficient when the resolution of the authorities changes within the field of view.

In a recent study published in Light: Science and Applications, a group of scientists introduced a new method called rolling Fourier ring correlation. This method helps to directly represent resolution heterogeneity in the super-resolution domain, achieving unparalleled SR scale mapping and easily associating resolution mapping with SR content. In addition, the team also improved the resolution scaling error map to achieve more accurate system error estimation. This is combined with rFRC to create a combination technique called PANEL, which focuses on accurately locating low reliability areas from SR images.

Scientists have successfully applied PANEL to various imaging methods, including single molecule localization microscopy, super-resolution radial wave, structural illumination microscopy, and deconvolution methods, verifying the effectiveness and stability of its quantitative spectra. PANEL can be used to improve SR images. For example, it has been effectively used to fuse SMLM images reconstructed through various algorithms, providing high-quality SR images.

Source: Laser Net

Related Recommendations

  • Probe organization of photoacoustic devices using low-cost laser diodes

    Photoacoustic technology provides a non-invasive method for detecting biological tissues, but its clinical application is limited, partly due to the large volume and high cost of laser sources. A compact PA sensing instrument powered by laser diodes for biomedical tissue diagnosis can provide clinical doctors with a practical and effective tool for evaluating breast diseases.By providing a cost-ef...

    2024-03-06
    See translation

  • Topcon Announces the Launch of LN-50 3D Laser

    Earlier this month, before the annual Intergeo conference held in Germany, Topcon Positioning Systems announced the latest member of its robot total station series. This California based company launched the LN-50 3D laser in early October, marking their latest layout navigator, which has a range of 50 meters.They pointed out that this latest scanner is specifically designed for homebuilders, mech...

    2023-10-25
    See translation

  • Acousto optic modulation of gigawatt level laser pulses in the ambient air of Nature Photonics

    An interdisciplinary research group, including the German synchrotron radiation accelerator DESY and the Helmholtz Institute in Jena, Germany, reported that invisible gratings made of air not only are not damaged by lasers, but also maintain the original quality of the beam. The relevant research has been published in Nature Photonics under the title of "Acousto opt modulation of gigawatt scale la...

    2023-10-12
    See translation

  • Germany has developed a fast, accurate, and wear-resistant laser drilling CFRP process

    Recently, scientists from the Hanover Laser Center (LZH) in Germany announced the successful development of an automated laser drilling process that can promote the processing of carbon fiber reinforced plastics (CFRP). They stated that this is particularly valuable in applications such as lightweight structures and sound insulation.Composite materials such as carbon fiber reinforced plastics (CFR...

    2024-03-06
    See translation

  • $75 million, this laser equipment manufacturer will be acquired

    Rocket Lab USA continues its path of vertical integration and has signed an exclusive but non binding agreement with MynaricAG, a German laser communication terminal (LCT) supplier and Rocket Lab supplier, to acquire the company for $75 million in cash or stock.If Mynaric achieves its revenue target, it will pay an additional revenue of up to $75 million. This acquisition depends on whether Myna...

    03-25
    See translation