chanelink logo
CHANELINK
English

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

1163
2024-03-29 15:03:26
See translation

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Related Recommendations

  • Scientists build high-power cladding-pumped Raman fiber laser in 1.2 μm band

    Laser sources operating in the 1.2 μm band have some unique applications in photodynamic therapy, biomedical diagnostics, and oxygen sensing. In addition, they can be used as pump sources for mid-infrared optical parameter generation and visible light generation through frequency doubling.Laser generation in the 1.2 μm band has been achieved by different solid-state lasers, including semicon...

    2024-01-31
    See translation

  • Application of Multipurpose Femtosecond Laser Interferometry in High Precision Silicon Nanostructures

    Researchers from the Laser Processing Group of the IO-CSIC Institute of Optics in Spain report on the application of multi-purpose femtosecond laser interference in high-precision silicon nanostructures. The related research was published in Optics&Laser Technology with the title "Versatile femtosecond laser interference pattern applied to high precision nanostructured of silicon".Highlights:...

    2024-07-10
    See translation

  • New progress in research on laser cleaning and improving the damage threshold of fused quartz components at Shanghai Optics and Machinery Institute

    Recently, the research team of the High Power Laser Element Technology and Engineering Department of the Shanghai Institute of Optics and Mechanics, Chinese Academy of Sciences, has made new progress in the study of improving the damage threshold of fused quartz elements through laser cleaning. The study proposes for the first time the use of microsecond pulse CO2 laser cleaning to enhance the dam...

    2024-07-08
    See translation

  • A New RIEGL Laser Scanning Solution for Drone Data Acquisition

    With its latest developments, RIEGL once again emphasizes its pioneering role as a supplier of high-performance LiDAR sensors and integrated systems with UAS. The continuous trend in the drone system industry requires measurement level laser scanners that match the integrated performance of compact multi rotor and high-speed vertical takeoff and landing or fixed wing drone platforms.RIEGL has reco...

    2023-12-01
    See translation

  • The scientific research team has proposed a modeless Raman fiber laser using a traditional resonant cavity structure

    The pump source, gain material, and resonant cavity are the three elements that make up a laser. Due to the selective effect of the resonant cavity on the lasing frequency, multi longitudinal mode operation is one of the characteristics of fiber lasers based on traditional resonant cavity structures, manifested as periodic beat peaks in the radio frequency (RF) spectrum and periodic fluctuations i...

    2023-08-15
    See translation