chanelink logo
CHANELINK
English

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

1299
2024-03-29 15:03:26
See translation

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Related Recommendations

  • Scientists have created a full spectrum white light laser with bright spot, smooth and flat spectrum, and large pulse energy characteristics

    Recently, the team led by Professor Li Zhiyuan from South China University of Technology has successfully developed a full spectrum white light laser, which has the characteristics of bright spot, smooth and flat spectrum, and large pulse energy. It can cover the ultraviolet visible infrared full spectrum of 300-5000nm, with a single pulse energy of 0.54mJ.The launch of such a full spectrum white ...

    2023-11-07
    See translation

  • Changing Optical Design: How Multi scale Simulation Improves the Efficiency of Modern Devices

    Optical equipment is an integral part of technologies such as data centers and autonomous vehicle, which are constantly developing to meet the needs of complex applications. The challenge faced by designers is to manipulate light at the wavelength scale to achieve the required optical properties, which requires precision at both the nano and macro scales. Nanoscale structures, such as those on LED...

    2024-03-02
    See translation

  • Mazak will showcase high-speed fiber lasers on Tube 2024

    Yamazaki Mazak designed the FT-150 fiber laser tube processing machine for high-speed cutting of small and medium-sized diameter pipes, for use in Tube 2024. The machine tool will be controlled by a new type of pipe cutting CNC, which will be exhibited for the first time in Europe.Tube 2024 will be held from April 15th to 19th in Dusseldorf, Germany. Mazak will be exhibited at booth C17 in Hall 5....

    2024-03-16
    See translation

  • 2026 SPIE Entrepreneurship Challenge Opens for Registration

    Applications are now open for the 2026 SPIE Startup Challenge. The annual entrepreneurial pitch competition is held by SPIE, the international society for optics and photonics, as part of SPIE Photonics West.In 2026, Photonics West will be held 17-22 January in San Francisco’s Moscone Center, with the SPIE Startup Challenge finals being held 20 January.The SPIE Startup Challenge is a competitive e...

    09-08
    See translation

  • China University of Science and Technology proposes composite cold field 3D printing technology for liquid crystal elastomers

    Recently, Associate Professor Li Mujun from the School of Engineering Sciences and the Institute of Humanoid Robotics at the University of Science and Technology of China, together with researchers such as Professor Zhang Shiwu, has made significant progress in the field of intelligent material 3D printing. The research team proposed composite cold field 3D printing technology and successfully pre...

    02-25
    See translation