chanelink logo
CHANELINK
English

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

1139
2024-03-29 15:03:26
See translation

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Related Recommendations

  • Micro ring resonators with enormous potential: hybrid devices significantly improve laser technology

    The team from the Photonic Systems Laboratory at the Federal Institute of Technology in Lausanne has developed a chip level laser source that can improve the performance of semiconductor lasers while generating shorter wavelengths.This groundbreaking work, led by Professor Camille Br è s and postdoctoral researcher Marco Clementi from the Federal Institute of Technology in Lausanne, represe...

    2023-12-11
    See translation

  • Van's updates the manufacturer of laser-cut parts

    Van's Aircraft has responded to reports of ruptured dented parts found in AirVenture's latest kit. These defects are caused by external suppliers changing the process of laser cutting parts. From February 2022 to June 2023, Van's moved some parts from traditional punch manufacturing to an outside supplier that can laser cut rivet holes. The move is designed to increase the company's throughput and...

    2023-08-04
    See translation

  • NUBURU Announces Second Next Generation Blue Laser Space Technology Contract with NASA

    NUBURU, the leading innovator of high-power and high brightness industrial blue laser technology, announced today that it has been awarded a second phase contract worth $850000 by the National Aeronautics and Space Administration (NASA) to advance blue laser power transmission technology as a unique solution that significantly reduces the size and weight of equipment required for lunar and Martian...

    2024-05-13
    See translation

  • Based on Transform Optics: Realizing an Ideal Omnidirectional Invisible Cloak in Free Space

    A team led by Professor Ye Dexin and Professor Chen Hongsheng from Zhejiang University, as well as Professor Yu Luo from Nanyang University of Technology, conducted practical research on full parameter transformation optical devices. The research team has designed and implemented an all parameter omnidirectional invisibility cloak based on the theory of linear transformation optics and omnidirecti...

    2024-04-29
    See translation

  • Professor Wu Dong's team at the University of Science and Technology of China created a "dancing microrobot" using femtosecond laser composite materials.

    It was learned from the University of Science and Technology of China that the team of Professor Wu Dong of the Micro and Nano Engineering Laboratory of the school proposed a femtosecond laser two-in-one multi-material processing strategy, manufactured a micromechanical joint composed of temperature-sensitive hydrogel and metal nanoparticles, and then developed a multi-joint humanoid micromachine ...

    2023-08-11
    See translation