chanelink logo
CHANELINK
English

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

1172
2024-03-29 15:03:26
See translation

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Related Recommendations

  • Panasonic Launches 3D Short Pulse Fiber Precision Laser Marking Machine LP-ZV

    Recently, Panasonic has launched the latest laser marking technology product - the LP-ZV series, which can provide high-precision and high-efficiency laser marking.Panasonic claims that the LP-ZV series has set a new standard that can bring excellent speed and accuracy in operation, suitable for various applications such as marking text, graphics, barcodes, and 2D code.The company stated that the ...

    2023-11-08
    See translation

  • Four ways researchers harness the power of lasers to achieve manufacturing excellence

    The use of industrial lasers has become a viable option for many manufacturing processes. It enables workers to simplify steps, improve precision and benefit from the benefits associated with output. Decision makers will get the best results when they consider the specific possibilities of using lasers in manufacturing. Here are some options.Improved cleaning and texturing methodsMany man...

    2023-08-04
    See translation

  • Chinese researchers enhance perovskite lasers by suppressing energy loss

    Limiting Auger recombination enables “record” quasi-continuous wave laser output.For years, engineers have sought better ways to build tiny, efficient lasers that can be integrated directly onto silicon chips, a key step toward faster, more capable optical communications and computing.Today’s commercial lasers are mostly made from III-V semiconductors grown on specialized substrates—a process that...

    08-25
    See translation

  • Accelerating electrons by emitting laser light into a nanophotonic cavity

    The laser driven particle accelerator on silicon chips was created by two independent research groups. With further improvements, this dielectric laser accelerator can be used in medicine and industry, and even in high-energy particle physics experiments.Accelerating electrons to high energy is usually accomplished over long distances in large and expensive facilities. For example, the electron ac...

    2023-10-28
    See translation

  • Scientists have conducted a series of studies on the mechanical properties and flame retardancy of laser formed Ti40 flame-retardant titanium alloy

    Recently, Professor Huang Chunping's team from Nanchang University of Aeronautics and Astronautics conducted a series of studies on the mechanical and flame retardant properties of laser formed Ti40 flame retardant titanium alloy. The research team used typical Ti40 flame-retardant titanium alloy as the research object and prepared Ti40 flame-retardant titanium alloy using LSF technology. The micr...

    2023-08-15
    See translation