English

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

1319
2024-03-29 15:03:26
See translation

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Related Recommendations
  • Super-resolution fluorescence microscopy utilizes fluorescent probes and specific excitation and emission programs

    Super-resolution fluorescence microscopy surpasses the diffraction limit of what used to be a barrier by using fluorescent probes and specific excitation and emission programs. Most SR technologies heavily rely on image computation and processing to retrieve SR information. However, factors such as fluorescence group photophysics, chemical environment of the sample, and optical settings may cause ...

    2024-01-23
    See translation
  • Photonic hydrogel of high solid cellulose with reconfigurability

    Recently, Qing Guangyan, a researcher team from the Research Group on Bioseparation and Interface Molecular Mechanism (1824 Group) of Biotechnology Research Department of Dalian Institute of Chemical Physics, Chinese Academy of Sciences, designed and prepared a highly solid cellulose photonic hydrogel with reconfigurability and mechanical discoloration. This preparation method opens up a new way t...

    02-17
    See translation
  • Lorenz competes in the LiDAR market with MEMS galvanometer technology

    At the recently concluded 2024 International Consumer Electronics Show (CES), automotive related technologies and solutions shone brightly, and a group of Chinese LiDAR suppliers competed on the same stage.The technologically advanced products, systematic solutions, continuously increasing delivery and market retention have to some extent proven that in the context of the development of automotive...

    2024-04-13
    See translation
  • Germany has developed a fast, accurate, and wear-resistant laser drilling CFRP process

    Recently, scientists from the Hanover Laser Center (LZH) in Germany announced the successful development of an automated laser drilling process that can promote the processing of carbon fiber reinforced plastics (CFRP). They stated that this is particularly valuable in applications such as lightweight structures and sound insulation.Composite materials such as carbon fiber reinforced plastics (CFR...

    2024-03-06
    See translation
  • Juguang Technology launches miniaturized high-power semiconductor laser stack GS09 and GA03

    In today's technology field, Juguang Technology released two highly anticipated high-power semiconductor lasers on December 13th: GS09 and GA03. These two products are leading the innovation wave in the laser industry with their miniaturized design, excellent thermal management capabilities, and extensive customization flexibility.GS09 revolutionizes chip spacing by compressing the width of the st...

    2023-12-15
    See translation