chanelink logo
CHANELINK
English

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

1338
2024-03-29 15:03:26
See translation

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Related Recommendations

  • Germany has developed a fast, accurate, and wear-resistant laser drilling CFRP process

    Recently, scientists from the Hanover Laser Center (LZH) in Germany announced the successful development of an automated laser drilling process that can promote the processing of carbon fiber reinforced plastics (CFRP). They stated that this is particularly valuable in applications such as lightweight structures and sound insulation.Composite materials such as carbon fiber reinforced plastics (CFR...

    2024-03-06
    See translation

  • Future oriented strategic technology: integrated manufacturing of large composite materials with additive and subtractive materials and its key elements

    Thermowood has developed a large-scale additive and subtractive material manufacturing equipment, LSAM, and successfully printed tooling molds on site that can be used for aerospace composite material forming, demonstrating its low-cost and rapid response to composite material manufacturing capabilities to the public.As a large-scale component additive manufacturer, Thermowood has developed a near...

    2024-04-19
    See translation

  • Manufacturing customized micro lenses with optical smooth surfaces using fuzzy tomography technology

    Additive manufacturing, also known as 3D printing, has completely changed many industries with its speed, flexibility, and unparalleled design freedom. However, previous attempts to manufacture high-quality optical components using additive manufacturing methods often encountered a series of obstacles. Now, researchers from the National Research Council of Canada have turned to fuzzy tomography (a...

    2024-05-30
    See translation

  • NLIGHT announces financial performance for the fourth quarter and full year of 2024

    Recently, nLIGHT, a manufacturer of high-power semiconductors and fiber lasers, announced its financial performance for the fourth quarter and full year of 2024.financial summaryTotal revenue: 198.5 million US dollars, a decrease from 209.9 million US dollars in 2023, due to a decline in sales in the laser product department.Operating loss: A loss of $65.6 million, compared to a loss of $46.8 mill...

    03-04
    See translation

  • Laser giant announces launch of new fiber laser platform

    Recently, Coherent Corp. announced the launch of the EDGE FL TM high-power fiber laser series, tailored specifically for cutting applications in the machine tool industry. The power levels of the EDGE FL series range from 1.5kW to 20kW, redefining the balance between value and performance to meet the growing demand for high-power, reliable laser sources in fiber laser cutting.With the increasing d...

    2024-10-23
    See translation