English

Optical properties of Xinggory Cy3.5 amine/NH2 labeling experiment

1148
2024-03-29 15:03:26
See translation

The optical properties of the Cy3.5 amine labeling experiment are an important reason for its application in biomarkers and fluorescence imaging. Cy3.5 is a fluorescent dye belonging to the Cyanine dye family, with high molar extinction coefficient and quantum yield, making it excellent in trace analysis and fluorescence imaging.

In the Cy3.5 amine labeling experiment, the dye covalently binds to specific functional groups on biomolecules (such as proteins, nucleic acids, etc.) through its amine group, thereby achieving the labeling of the target molecule. This labeling not only maintains the biological activity of the target molecule, but also endows it with fluorescence characteristics, making it convenient for qualitative and quantitative analysis in complex biological samples.

The maximum excitation wavelength of Cy3.5 dye is usually in the range of 550-570nm, while the maximum emission wavelength is in the range of 570-590nm. This gives Cy3.5 dye unique emission characteristics between green and red fluorescence, enabling good spectral separation from other commonly used fluorescent dyes such as GFP, FITC, etc., avoiding signal interference.

In addition to fluorescent signals with high sensitivity and specificity, Cy3.5 dyes also exhibit good photostability. Under continuous laser irradiation, its fluorescence signal can remain relatively stable and is not prone to bleaching or quenching. This makes Cy3.5 dye have better application prospects in long-term fluorescence imaging experiments.

In summary, the optical properties of the Cy3.5 amine labeling experiment make it a tool in the fields of biomarkers and fluorescence imaging. Its high sensitivity, specificity, good spectral separation, and excellent photostability make this dye valuable in biomedical research.

Source: Sohu

Related Recommendations
  • Single photon avalanche diode detector enables 3D quantum ghost imaging

    A team of researchers at the Fraunhofer Institute for Optoelectronics, Systems Technology and Image Development and Karlsruhe Institute of Technology are using single-photon avalanche diode (SPAD) arrays to achieve three-dimensional (3D) quantum ghost imaging.The new method, called "asynchronous detection," produces the lowest photon dose of any measurement and can be used to image light-sensitive...

    2023-09-06
    See translation
  • Coherent and Faraday sign a partnership to expand the manufacturing scale of high-temperature superconducting (HTS) tapes

    Recently, American photonics giant Coherent and Japan's Faraday 1867 Holdings signed a Letter of Intent (LOI), with the goal of expanding the manufacturing scale of high-temperature superconducting (HTS) tapes to be widely used in large-scale deployment of nuclear fusion reactors, while also promoting the transformation of green energy. Coherent's excimer laser is expected to be more widely used i...

    2023-10-12
    See translation
  • High performance optoelectronic device developer "Micro Source Photon" completes B+round financing

    Recently, Weiyuan Photon (Shenzhen) Technology Co., Ltd. (hereinafter referred to as "Weiyuan Photon") announced the completion of a B+round of financing, with investors including Yicun Capital, Chenfeng Capital, and Beijing Guoqian Investment. The specific amount has not been disclosed. According to its official website, MicroSource Photonics was founded in November 2018, with the main members...

    2024-07-23
    See translation
  • 330 million US dollars! This laser ophthalmic treatment developer has been acquired

    Recently, according to a report submitted by BioLight to the Tel Aviv Stock Exchange, Swiss American pharmaceutical and medical device giant Alcon Pharmaceuticals is acquiring Israeli medical technology company Belkin Vision.It is reported that BioLight will sell its 4% stake in Belkin Vision, which may be worth up to $330 million based on the milestones established in the transaction.Belkin Visio...

    2024-05-06
    See translation
  • The rare decay of the Higgs boson may point to physics beyond the standard model

    Particle physicists have detected for the first time a new decay of the Higgs boson, revealing subtle differences predicted by the standard model and potentially pointing to new physics beyond it. The research results are published in the journal Physical Review Letters.The theoretically predicted Higgs boson since the 1960s was finally discovered in the European CERN laboratory in 2012. As a quan...

    2024-01-26
    See translation